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KMID : 0352819960110010009
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Kosin Medical Journal
1996 Volume.11 No. 1 p.9 ~ p.18
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Expression of c-myc gene in benzo(a)-pyrene-poisoned human lymphcblast NC-37 cells
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Abstract
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To investigate expression in c-myc gene by a chemical carcinogen benzp(a)pyrene(BP), human lymphoblast NC-37 cells were exposed to varjous concentrations of BP and the c-myc gene expression was evaluated by northern and slot blot hybridization
methods. When NC-37 cells exposed to BP of 0-4 g/ml were treated with HindII/XbaI restriction enzyme, the c-myc genes were cut at the same region regardless of BP concentration. The site of cleavage by the restriction enzyme, HendII/Xbal
was
identical in the control, BP-treated and BP-washed cells. However, the m-RNA cxpression in slot blot hybridization appeared to be 4-5 times higher in BP-treated cells than in the control, and this effect was partially removed by
washing
the BP. When the DNA isolated from NC-37 cells exposed to various concentrations(0, 2, 4 g/ml) of BP were amplified by polymerase chain reaction using a primer containing c-myc exon I, the resulting DNA were of the same size in
all
groups. These results suggest that overexplession of c-myc oncogene may be required for the malignant transformation and maintenance in benzo(a)pyrene poisoned human lymphoblast NC-37 cells.
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KEYWORD
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